The Research Project on Microbiology

Question: Explain Microbiology by Preaparing biofilm cultures using E.coli inoculum? Answer: Research Project: Preparation of biofilm cultures using E.coli inoculum. Investigation of antibiotic tolerance of bacterial biofilms and compared it with planktonic cells. GFP transformation of bacteria is carried out and for the purpose of fluorescent microscopic observation of bacteria. Data analysis and interpretation from observation of streak platting, serial dilution series counting and bacterial transformation data. Laboratory Experience: Skills and Techniques: Microbiology: Biofilms assays, microbiological aseptic technique, streak and spread plating, dilution series plating, gram stain, light field microscopy, epifluorescence microscopy, media and reagents preparations, plate pouring, simple stain, selective media and biochemical characterisation of bacteria, work with fungi and viruses, analysis and interpretation of control and experiment such as counting of bacteria, data for calculations of transformation efficiency, microscopic observations of pGLO GFP fluorescent cells, biofilm culture inoculum, fluorescence microscopy, backlight Live/dead stain, microscopic evaluation of antibiotic treated biofilm culture and planktonic cells. Biochemistry: protein denaturation and hydrolysis, HPLC, DNA isolation (plasmid and chromosomal), genetic colour tagging, bacterial transformation with GFP, agarose gel electrophoresis, western blotting, northern blotting, paper chromatography, affinity chromatography, enzyme kinetics, and PCR. Others: handling of animals and bacterial cultures, handling of instrumentation such as UV and IR. Work Experience: January, 2014 to present Currently working as receptionist at city life education and action for refugees. May 2008- September 2009 Pharmacy Counter Assistant at S.K. Roy dispensing chemist, Ltd. June 2007- November 2008 Sales Assistant at Oxfam music store at Southhampton. June 2007- December 2007 Hedge end waitress at Marks Spencer restaurant. As research on microbial biofilms is going on in various topic, which focus on the elucidation of the related to biofilm microorganisms. Evaluation of control process which includes medical devices treated with antimicrobial agents, for prevention of the biofilm colonization of medical devices such as catheter, and to investigate new processes which increase efficiency to control growth of biofilm and associated diseases with them. The field of microbiology has begun to accept the concept of the biofilm phenotypes. Researchers has begun to investigate microbiologic processes in the field of food, water, clinical materials, from a biofilm prospective. As my interest in biofilms and have done project in this field, a desire is created, to understand the role of biofilms in chronic diseases which are associated with medical devices such as catheter or role of biofilms in antimicrobial resistance or biofilms as a reservoir for pathogenic organisms. I am applying for this project, because this research project, fulfil all my requirements and help me to understand and develop my skills about biofilm formation on catheter and their association with chronic diseases, on which I always wanted to work and learn, about the role and behaviour of biofilms in chronic diseases and resistance against antibiotics. References: Bjarnsholt T. 2013. The role of bacterial biofilms in chronic infections. APMIS Suppl. (136):1-51. Donlan R.M. 2001. Biofilms and Device-Associated Infections. Emerging Infectious Diseases. 7(2): 277-281. Donlan R.M. 2002. Biofilms: Microbial Life on Surfaces. Emerging Infectious Diseases. 8(9): 881-890. Mary A. 2013. Role of Bacterial Biofilms in Catheter-Associated Urinary Tract Infections (CAUTI) and Strategies for Their Control. https://www.intechopen.com/books/recent-advances-in-the-field-of-urinary-tract-infections/role-of-bacterial-biofilms-in-catheter-associated-urinary-tract-infections-cauti-and-strategies-for-.